用基于PCR的分子标记分析甘蓝型油菜抗感菌核病品系间的遗传距离

用离体叶接种法鉴定了甘蓝型油菜选系对菌核病的抗感性，结果表明绝大多数抗性品系与感病品种间的差异达到显著或极显著水平。以两种基于ＰＣＲ的标记估计所选１０ 份材料间的遗传距离。１９ 个随机引物扩增出了２６ 条重复性很好的ＲＡＰＤ 标记，１ 对依据植物Ｒ基因的ＮＢＳ区段设计的简并引物扩增出了３ 条多态性的带。依据２９ 个标记进行聚类分析，结果表明抗、感性材料在遗传距离为０．１２０６ 处被明显地区分开。５份抗性材料中，宁ＲＳ—１ 与中油８２１、中Ｒ８８８ 等其它抗性品系的遗传距离较远     

四种暖季型草坪草对SO2的抗性及净化能力的比较

 本实验采用人工模拟熏气法，以ＣＫ（自然状态）、Ｓ１（１．５ ｍｇ／Ｌ）、Ｓ２（２．２５ ｍｇ／Ｌ）、Ｓ３（３ ｍｇ／Ｌ）、Ｓ４（３．７５ ｍｇ／Ｌ）５个ＳＯ_２ 浓度水平，对４种暖季型草坪草——假俭草、结缕草、百喜草、狗牙根进行密闭熏气处理；进而测定４种草坪草生理生化指标；最终比较４种草坪草对ＳＯ_２ 的抗性和净化能力。结果表明，４ 种草坪草叶绿素总量随ＳＯ_２ 浓度增加呈降低趋势；可溶性糖、丙二醛、脯氨酸、超氧化物歧化酶、过氧化氢酶、过氧化物酶、叶片硫含量则随ＳＯ_２ 浓度增加呈升高趋势；可溶性蛋白除狗牙根降低外其余均呈升高趋势。采用模糊数学隶属度公式进行综合相关分析得出，４种草坪草对ＳＯ_２ 的抗性比较：狗牙根＞ 结缕草＞ 百喜草＞ 假俭草；净化能力比较：结缕草＞ 百喜草＞狗牙根＞假俭草。     

《牧草栽培学》课程整体教学改革与实践

通过时<牧草栽培学>课程教学内容、教学方法、教学手段和教学实习等方面的投入和研究,提出了完整的<牧草栽培学>教学体系和改革方案.进而提高<牧草栽培学>的课堂教学质量.     

观赏竹容器青苗基质开发初步研究

国际市场中要求竹苗的出口不能带土,根据基质选择的原则及评价的标准,配制了8种基质(G1、G2、G3、G4、G5、G6、G7、G8)作为鸡毛竹(S.chinensis Nakai)和黄条金刚竹(Pleioblastus kongosanensis f.aureostriaus)的容器育苗基质.结果表明:在G8中,鸡毛竹、黄条金刚竹的生长情况最好,在G3、G7中,鸡毛竹、黄条金刚竹的生长情况较好,但成本相对较高.在泥炭中加入山核桃壳或煤渣等经济基质材料,降低了成本,改善了基质的通气性和保水性.     

Metformin对蛋鸡AMPK活性及脂质代谢的短期效应研究

为了研究双胍药类Mefformin短期内对蛋鸡AMPK活性的影响,研究选用日龄、体重及产蛋率相近的产蛋高峰期新罗曼蛋鸡40只,随机分为4个处理组,每个处理5个重复,每个重复2只鸡,对照组蛋鸡采食不合Mefformin的基础饲粮,分别在另外3组蛋鸡基础饲粮中添加0.6%、0.8%、1.0%Metformin.试验结果表明:不同浓度的Metformin处理呈剂量效应性地显著降低蛋鸡的采食量;肝脏AMPK基础生理活性比卵巢AMPK活性高46.89%(P=0.124),0.6%、0.8%、1.0%Mefformin组蛋鸡肝脏AMPK活性分别增加了13.83%(P=0.216)、25.90%(P=0.162)、31.10%(P=0.170);血清三酰甘油(TG)、极低密度脂蛋白胆固醇(VLDL-C)受1.0%Mefformin处理影响最大,分别下降了27.10%(P=0.256)、26.75%(P=0.220);各处理组蛋鸡卵泡胆固醇含量均显著或极显著地低于对照组(P＜0.05或P＜0.01),且随Metformin浓度的增加呈剂量依赖性地降低.结果说明0.6%～1.0%Mefforrain提高了蛋鸡肝脏AMPK活性,并降低了蛋鸡血清脂质合成产物及不同发育程度卵泡胆固醇的含量.     

Effect of simvastatin on expression of integrin-linked kinase and renal tubulointerstitium injury in rats induced by high fat diet

AIM: To explore the effect of simvastatin on expression of integrin-linked kinase (ILK) and the injury of renal tubulointerstitium in the rats induced by high fat diet. METHODS: Fifty-four 6-8 week-old female Wistar rats were randomly assigned to the following three groups: high fat diet group, simvastatin group (rats were fed with high fat diet plus 10 mg·kg-1·d-1 simvastatin) and control group. Six rats in each group were sacrificed at 4th week, 10th week, and the others at 20th week. The injury of renal tubulointerstitium was observed under microscope with HE staining and the expression of renal ILK was determined by Western blotting analysis and immunohistochemistry. Levels of total cholesterol (TC) and triglyceride (TG) in serum were measured by enzymatic colormetric methods. RESULTS: The serum TC and TG levels and the expression of renal ILK significantly increased in both high-fat diet group and simvastatin group, compared to control group at 4th week, reaching a maximum at 20th week (P<0.01). Tubulointerstitium injuries including vacuolar degeneration, syncytial change, clody swelling, necrosis and atrophy of renal tubular epithelial cells, thinning of tubal wall, lumens compensational expansion or even abolition, and inflammatory cell infiltration, interstitial fibrosis were found in both high fat diet group and simvastatin group, compared to control group at 4th week, worsened to a maximum at 20th week. However, all of these ameliorated in simvastatin group, compared to high fat diet group at each time point. CONCLUSION: The results indicate that high-fat diet induces significant lesion of renal tubulointerstitium and increased expression of renal ILK. Simvastatin may play an important role in protecting against tubulointerstitium injury induced by hyperlipoidemia by down-regulating the expression of renal ILK.     

我国农业植保网络信息服务的发展前景

本文阐述了农业植保网络信息服务在我国农业应用的现状和前景，分析了农业信息化在我国的发展意义，讲述了构建交互式、智能化的植保网络信息服务的应用，对实现跨世纪农业的发展具有重要意义。     

实行借书逾期罚款制度涉嫌侵权

作者根据国家的有关法律法规 ,提出了图书馆实行借书逾期罚款制度无法律依据 ,超越了有关规定 ,是一种侵权行为 ;同时对做好读者服务工作 ,加强图书馆规章制度的宣传教育方面谈了自己的看法。     

国内高校图书馆学科导航建设现状调查与分析

通过对国内183所高校图书馆学科导航建设情况的调查，概述了目前国内高校图书馆学科导航的建设现状、服务模式和服务内容，并分析了其中存在的问题，提出了一些对策。